Dolly Parton due to dominate Glastonbury
Parton also tweeted earlier on Sunday: "We promise y'all a Tennessee welcome!"
Dolly Parton says she's written a song about the mud especially for her Glastonbury debut.
The country singer and cultural icon is due to take the stage at 16:20 BST, and says she'll play for just over an hour.
" I grew up in mud," she told reporters in a pre-show press conference.
"My daddy was a farmer, I grew up in East Tennessee and we made our living on a farm, so I thought, 'Well this isn't all that different'."
She added: "Mud is mud, wherever you are."
Fans will be expecting hits including 9 to 5, I Will Always Love You and Coat of Many Colors.
The English National Ballet made their debut Glastonbury performance earlier on Sunday
Festival-goers came well prepared for the mudbath
Kasabian are headlining the festival on Sunday evening
But the 68-year-old said she had struggled to write the set list."It was kinda tricky knowing what to do under a festival situation because in my show, as a rule, I usually do a couple of hours but they said, 'You can't do but an hour, maybe an hour and ten.'
"So I thought, I can't do a bunch of sad, slow songs because everyone's drunk and high.
"There's two or three songs they have to hear, like Coat of Many Colors. But we've tried to do a set where we can keep it movin' pretty good, and not bring 'em down too much."
A flashmob, or "Dollymob" as it is being called, has been planned for her performance of Jolene.
Fans have been encouraged to learn the simple dance routine, which Glastonbury organisers posted on YouTube last month.
The singer also revealed she would bring out Bon Jovi guitarist Richie Sambora to play a cover of Lay Your Hands On Me during her set.
Prior to her show, the singer was presented with a special award in recognition of selling more than 100 million records worldwide. The singer said she would hang it in her Dollywood Museum in Tennessee.
Her performance has been the talk of the weekend, with several performers jokingly threatening to abandon their performances on other stages to catch the singer in action.
Bands who are lucky enough not to be in competing slots have all said they intend to catch the show.
"I think she's an amazing writer, and I'd love to see her in the flesh," said Yukimi Nagano of Little Dragon.
Even Lars Ulrich, of Saturday night headliners Metallica, plans to take it in. "I'll have a hangover on Sunday but I want to see The Horrors, The Black Keys and Dolly Parton," he told The Mirror.
"I'm a big fan. We all bow to the altar of Dolly."
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